High throughput phenotyping in plants refers to the rapid and automated measurement of various phenotypic traits in a large number of plants. Phenotyping involves the assessment of observable characteristics or traits of plants, such as growth patterns, morphology, physiology, and responses to environmental stimuli. High throughput phenotyping aims to accelerate the data collection process, enabling researchers and plant breeders to analyze large populations more efficiently. Here are some key aspects of high throughput phenotyping in plants:

  1. Automation: High throughput phenotyping relies on automated systems to collect data quickly and accurately. This can include robotics, sensors, cameras, and other technologies that streamline the process of measuring multiple traits simultaneously.
  2. Imaging Technologies: Advanced imaging technologies play a crucial role in high throughput phenotyping. These may include high-resolution cameras, multispectral and hyperspectral imaging, infrared thermography, and 3D imaging. These technologies allow researchers to capture detailed information about plant characteristics without manual intervention.
  3. Sensor Technologies: Various sensors are used to measure specific plant traits, such as chlorophyll content, leaf temperature, stomatal conductance, and nutrient levels. These sensors provide real-time data, contributing to a comprehensive understanding of plant performance.
  4. Data Analytics and Machine Learning: The massive amount of data generated through high throughput phenotyping requires sophisticated data analytics tools and machine learning algorithms. These technologies help in extracting meaningful patterns, identifying correlations, and making predictions based on the collected data.
  5. Phenotypic Traits: High throughput phenotyping can be applied to various phenotypic traits, including plant height, leaf area, flowering time, stress tolerance, disease resistance, and nutrient efficiency. Researchers can customize their phenotyping approach based on the specific traits relevant to their objectives.
  6. Field Phenotyping: While much high throughput phenotyping is conducted in controlled environments such as growth chambers or greenhouses, there is a growing interest in field phenotyping. This involves using mobile platforms, drones, or other technologies to assess plant traits in the field, providing more realistic and applicable data.
  7. Genomic Integration: High throughput phenotyping is often combined with genomic data to understand the genetic basis of observed traits. This integration helps in identifying genes associated with desirable traits, facilitating the breeding of improved plant varieties.
    Applications of high throughput phenotyping in plants include crop improvement, precision agriculture, understanding plant responses to environmental stresses, and accelerating the breeding process. Overall, high throughput phenotyping in plants has revolutionized the field of plant biology and agriculture by enabling researchers and breeders to efficiently characterize and select plants with desired traits for further development and cultivation.
    Source: https://www.frontiersin.org/articles/10.3389/fpls.2015.00619/full

Dr. Md. Monirul Islam
Senior Scientist
ASRBC, ACI Seed

Fig: High-throughput plant phenotyping and data accumulation.