A 127-post collection

CRISPR Interference (CRISPRi) for Gene Suppression

CRISPR interference (CRISPRi) is a powerful technique derived from the CRISPR-Cas9 system, which originally evolved in bacteria as a defense mechanism against invading viruses. CRISPRi allows for precise control over gene expression by using a modified Cas protein (usually Cas9) to target specific DNA sequences and inhibit gene transcription without »

Enhanced Genome Editing Benefits from Magnetofection

Magnetofection is a technique that combines magnetic nanoparticles (MNPs) with nucleic acids (such as plasmid DNA, siRNA, or other genetic material) to facilitate their delivery into target cells using an external magnetic field. While magnetofection is primarily used for gene delivery, it can also offer several benefits for genome editing »

Site Directed Nucleases (SDN) for Targeted Genome Modification

SDN is the collective term for the technique of creating a specific break in DNA with a nuclease, an enzyme that cuts DNA. Targeted modifications to the plant's traits are intended to be introduced by using the DNA break and the plant's inherent repairing systems. Site-directed nucleases (SDNs) are a »

Introduction to CRISPR Cas 13: What Is It and What Can It Do?

CRISPR protein Cas 13 is responsible for cutting RNA. Using a guide RNA, which is a programmable string of nucleotides consisting of around 30 bases, Cas 13 can navigate its way to a particular RNA sequence. These nucleotides are complementary to those that are present in the targeted section of »

Plant Phenotyping: Past, Present, and Future

Plant phenotyping is the study of observable traits or characteristics of plants, encompassing their morphology, physiology, and behavior. This field plays a crucial role in understanding plant growth, development, and responses to environmental stimuli. Here's an overview of plant phenotyping in terms of its past, present, and future: Past: Manual »

Transcriptome Profiling of Abiotic Stress in Plants

Transcriptome profiling is a powerful approach to understanding the molecular responses of plants to various environmental stimuli, including abiotic stress. Abiotic stress factors such as drought, salinity, extreme temperatures, and nutrient deficiency can significantly impact plant growth and development. Analyzing the transcriptome, which represents the entire set of RNA transcripts »

ChIP-Seq data to speed up the progress of plant breeding

ChIP-Seq data plays a crucial role in plant breeding by providing valuable insights into the regulatory mechanisms that govern gene expression and influence various traits. Here are several ways in which ChIP-Seq data is important for advancing plant breeding: Identification of Regulatory Elements: ChIP-Seq allows the identification of regulatory elements »